LASER MICROBEAM CELL SURGERY AS A NOVEL METHOD OF GENE TRANSFER IN WHEAT

A MICRO-LASER BEAM (1ΜM ) HAS BEEN USED SUCCESSFULLY TO PUNCTURE THE CELL WALL AND CELL MEMBRANE OF EGYPTIAN WHEAT CALLI . USING THE OSMOTIC PRESSURE GRADIENT WE COULD TRANSFER SOME GENES TO THE CELLS THROGH THE INTRODUCED HOLES . WE CONFIRMED THE TRANSFER AND EXPRESSION OF GENES AND ITS STABILITY USING THE ANALYTICAL GENETIC TECHNIQUES AS (PCR) AND (RT-PCR) . WE COULD DEAL WITH ABOUT 1 MILLION CELLS IN 120 MINUTES USING THE INTRODUCED HERE NOVEL TECHNOLOGY