RECOMBINANT DNA FOR A VACCINE AGAINST BOOPHILUS ANNULATUS

THE ABSTRACT IN ENGLISH LANGUAGE (NOT MORE THAN 100 WORDS) IN OUR PREVIOUS RESEARCH, WE HAVE DOCUMENTED THE DEVELOPEMENT OF ANTI-TICK IMMUNE RESPONSES BY CATTLE HEAVILY INFESTED FOR A LONGTIME WITH THE EGYPTIAN B. ANNULATUS NATURAL PARASITE OF FARM ANIMALS IN EGYPT. ANTIBODIES MEASURED BY ENZYME LINKED IMMUNO SORBENT ASSAY (ELISA) TO SPECIFIED GLYCOPROTEIN ANTIGENS (66 KD AND 150 KD) EXTRACTED FROM B. ANNULATUS SALIVARY GLANDS AND GUTS THEN FRACTIONATED BY POLY ACRYLAMIDE GEL ELECTROPHORESIS. IN ADDITION, THESE GLYCOPROTEIN ANTIGENS WERE RECOGNIZED BY CELL-MEDIATED IMMUNE RESPONSE TESTS. THESE HUMORAL AND CELL MEDIATED IMMUNE RESPONSES WERE CORRELATED WITH THE DEGREE OF RESISTANCE TO BANNULATUS INFESTATION, IN A DECREASING ORDER BOS INDICUS, BOS TAURUS X BOS INDICUS CROSSES, THEN BOS TAURUS. SEARCH FOR THE DNA CODING FOR THESE IMMUNOGENIC ANTIGENS WAS STARTED. SEQUENCES WERE GENERATED BY POLYMERASE CHAIN REACTION TO COVER THE EXPECTED CODING DNA GENOME. FURTHER EXPERIMENTS TO IDENTIFY THE SPECIFIC DNA SEQUENCES ARE ONGOING. THE FINAL GOAL IS TO DEVELOP R-DNA FOR THE PRODUCTION OF FUNCTIONAL IMMUNOGENIC PROTEINS THAT COULD STIMULATE ANTI-TICK INFESTATION IMMUNITY